Our new Clear+ tissue clearing method is the only real strategy that delipidates samples without having adjust in morphology and with small influence on structural integrity.
Megatome is a vibrating microtome made to area a wide variety of samples, from organoids and biopsy samples to expanded rodent brains and intact human organs. With significant blade vibrating frequency and minimized blade deflection, Megatome permits high-throughput tissue sectioning with uniform surface profile, in addition to nominal tissue hurt and data decline.
Antibodies could get weeks to diffuse by means of just a few millimeters of tissue, using a steep labeling gradient from surface area to core.
Functioning the CLI straight from a Gradle undertaking will not be at the moment supported. A distribution have to be designed through gradlew :j2d-cli:distZip to create a zip file containing every thing needed to run.
Clone the project or seize the newest release. Operating the utility will vary a tiny bit determined by how you retrieve the undertaking.
Our preformulated EasyIndex Answer raises and homogenize the refractive index of delipidated tissue samples, rendering them fully clear. This permits mild penetration into the sample and makes sure the acquisition of significant-resolution, in-target picture knowledge.
Megatome is often a novel microtome that permits for megatomi.com top-precision sectioning of a wide array of tissue samples – from organoids, to arrays of animal organs, to intact human Mind hemispheres – with minimal tissue problems and data decline.
It’s time to update your microtome to Megatome. With exact high-frequency slicing for an unmatched choice of sample sizes and kinds – from organoids and tumors to expanded tissues, sample arrays, and intact primate organs – Megatome is optimized for numerous purposes.
The SmartSPIM mild sheet microscope delivers unparalleled resolution and acquisition speed in the course of intact tissue volumes, although its modular structure permits you to enhance and personalize unique components.
Docset generation demands at least two choices: the title of the docset and The placement of your Javadoc information to incorporate within the docset.
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SHIELD avoids the variability of hydrogel embedding and the knowledge decline from PFA preservation, protecting specimens for several rounds of processing.
Leverage the Distinct+ tissue clearing approach, in addition to eFLASH and patented stochastic electrotransport systems, to promptly apparent and label whole organs. Crucial highlights and characteristics include things like:
This plugin applies the java plugin into the challenge It is really run beneath. Which means in a very multi-module challenge, a top level job named javadoc can't be designed to mixture the